Shrooms and Drug Testing in 2025: What CDL Drivers, Employees & Students Should Know

As the conversation around psychedelics and wellness grows in the U.S., more people—from truck drivers to college students—are asking one thing:
What happens if I take shrooms and get drug tested in 2025?

Whether you’re working in a safety-sensitive role, applying for a job, or attending school, here’s everything you need to know about shrooms and drug testingin 2025.

What Are Shrooms, and Why Is Psilocybin a Concern?

Shrooms (aka magic mushrooms) contain psilocybin, a hallucinogenic compound. While some states have moved toward decriminalization or therapeutic use, psilocybin remains illegal at the federal level.

This matters for:

• CDL drivers

• DOT-regulated employees

• University students subject to random testing

• Job applicants for federal or private roles

Shrooms & CDL Drivers: DOT Still Says “No”

If you’re a CDL driver, you fall under DOT drug testing regulations (49 CFR Part 40). The standard 5-panel test includes:

• Marijuana

• Cocaine

• Opiates

• Amphetamines

• PCP

❌ Psilocybin is NOT part of the standard DOT test
✅ But if you’re suspected of being under the influence or self-report usage, your employer may:

• Order an expanded test

• Refer you for a SAP evaluation

• Require a full Return-to-Duty (RTD) program

Employees: What Private Employers Are Testing For

Private employers vary, but in 2025, many are:

• Expanding panel tests to include substances like psilocybin

• Updating drug-free workplace policies to include psychedelics

• Performing random, pre-employment, or post-incident testing

🧠 Even if shrooms aren’t detected by default, some companies use extended drug screening panels—especially in healthcare, aviation, and defense industries.

Students & Athletes: Are Shrooms Being Tested in Schools?

For students, particularly those in college athletics, scholarship programs, or federally funded schools, shroom use can still carry consequences.

• NCAA and school codes may include psychedelic substances

• Students involved in accidents or misconduct may face drug screening

• Some high schools now use 10-panel tests for behavioral concerns

💡 Important: Even if not tested, admission of use or visible impairment can lead to expulsion, suspension, or required treatment.

Detection Windows for Psilocybin

Can Shrooms Be Detected in 2025?

Yes, but not always.

Most people won’t get caught unless:

• The test is expanded to include psilocybin

• They’re in a DOT role and suspected of use

• They admit usage or show impairment

What Happens If You’re Caught?

Depending on your role, possible outcomes include:

• ✅ SAP Evaluation (for DOT drivers)

• ✅ Mandatory counseling or treatment

• ❌ Termination of employment

• ❌ Disciplinary action or loss of student privileges

👉 Need a SAP Evaluation to Return to Work? Start Here »

Legal ≠ Permitted at Work or School

Even if psilocybin is legal in Oregon, Colorado, or your city:

• It’s still illegal federally

• Employers and schools are not required to allow it

• Shroom use = policy violation in most testing environments

Final Takeaway: Shrooms and Drug Testing in 2025